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The resulting precipitate was centrifuged at 10,000 g for 40 min at 4 °C, washed with 10 mL of ice-cold acetone to remove residual TCA, and then either air-dried and resuspended in 0.2 mL of 0.05% aqueous acetic acid for subsequent analysis
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In this context, supplemental choline (250–500mg via Lipo B injection) prevents deficiency-driven hepatic lipid accumulation
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